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  4. malE Recombinant Monoclonal Antibody

malE Recombinant Monoclonal Antibody

  • 中文名稱：
    malE重組抗體
  • 貨號：
    CSB-RA846828A0ENV
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in Escherichia coli lysate
      All lanes: malE antibody at 1: 1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 44 kDa
      Observed band size: 44 kDa
  • 其他：

產品詳情

  • 產品描述：
    CSB-RA846828A0ENV malE重組單克隆抗體是一款經ELISA及Western Blot（WB）驗證的高特異性科研工具，靶向細菌麥芽糖結合蛋白（MalE/MBP）。該抗體通過重組表達技術制備，可精準識別原核表達系統中表達的MalE蛋白，適用于檢測帶有MBP標簽的融合蛋白及分析細菌麥芽糖轉運相關機制。在驗證實驗中，抗體在WB中呈現單一清晰的目標條帶，且ELISA檢測顯示線性范圍寬泛、靈敏度優異，適用于低豐度樣本分析。其應用場景涵蓋分子生物學研究中重組蛋白純化工藝的質控、原核表達系統蛋白表達水平的檢測，以及微生物代謝途徑相關蛋白的功能研究。產品經嚴格質檢，具有批次穩定性和高重復性，可為細菌蛋白互作、原核表達體系優化等基礎研究提供可靠支持。
  • Uniprot No.：
  • 基因名：
    malE
  • 別名：
    Maltose-binding periplasmic protein (MBP) (MMBP) (Maltodextrin-binding protein), malE
  • 反應種屬：
    Escherichia coli
  • 免疫原：
    A synthesized peptide derived from Escherichia coli malE
  • 免疫原種屬：
    Escherichia coli (strain K12)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    10F2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.
  • 基因功能參考文獻：
    1. Upon refolding, MBP initially collapses into a dynamic molten globule-like ensemble, then forms an obligatory on-pathway native-like folding intermediate that brings together sequentially remote segments and then folds globally after a long delay. PMID: 29295923
    2. Our current understanding of what makes MBP such an effective solubility enhancer for other proteins and how it facilitates the proper folding of its fusion partners is presented [REVIEW] PMID: 28132493
    3. Maltose Binding Protein is key to the coupling of maltose transport to ATP hydrolysis in vivo, because it facilitates the progression of the MalK dimer from the open to the semi-open conformation, from which it can proceed to hydrolyze ATP PMID: 26268698
    4. activates mouse macrophages and polarizes them into M1 macrophages PMID: 24825603
    5. a salt-bridge motif in the maltose-binding protein has a role in the initial steps of substrate transport PMID: 16143635
    6. Our findings indicate that the solubility-enhancing activity of MBP is mediated by its open conformation and point to a likely role for the ligand-binding cleft in the mechanism of solubility enhancement. PMID: 17964542
    7. These data indicate that unfolding of the mature domain of preMBP is likely not a rate-determining step in translocation when the protein is targeted to the translocase via SecB. PMID: 18241889
    8. tat mutants are able to transport maltose in the absence of malE. PMID: 18385983

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  • 亞細胞定位：
    Periplasm.
  • 蛋白家族：
    Bacterial solute-binding protein 1 family
  • 數據庫鏈接：