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Rat myelin basic protein(MBP) antibody ELISA Kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

大鼠髓鞘堿性蛋白(MBP)抗體酶聯(lián)免疫試劑盒（CSB-E17399r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的MBP Ab含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于神經(jīng)退行性疾病模型研究、神經(jīng)損傷修復(fù)機制探索以及藥物開發(fā)過程中MBP生物標(biāo)志物的動態(tài)監(jiān)測。支持科研人員高效開展神經(jīng)科學(xué)基礎(chǔ)研究、病理模型評估或神經(jīng)保護劑篩選等實驗，為揭示MBP在神經(jīng)生物學(xué)中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MBP Ab
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MBP antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-97
1:2	Average %	95
	Range %	89-99
1:4	Average %	90
	Range %	81-97
1:8	Average %	95
	Range %	91-100

回收率：

The recovery of rat MBP antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	95	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.