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  4. Human myelin basic protein(MBP) antibody ELISA Kit

Human myelin basic protein(MBP) antibody ELISA Kit

  • 中文名稱：
    人髓鞘堿性蛋白(MBP)抗體酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04787h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人髓鞘堿性蛋白(MBP)抗體酶聯(lián)免疫試劑盒（CSB-E04787h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、腦脊液（CSF）樣本中的MBP Ab含量。人髓鞘堿性蛋白（MBP）抗體是一種針對(duì)髓鞘堿性蛋白的自身免疫抗體，與多發(fā)性硬化癥（MS）等脫髓鞘疾病的發(fā)生密切相關(guān)。研究顯示，MBP抗體在神經(jīng)組織細(xì)胞膜上裂解MBP，導(dǎo)致髓鞘損傷，進(jìn)而引發(fā)MS。目前，針對(duì)MBP抗體的研究主要集中在揭示其與MS等疾病的關(guān)系，以及開發(fā)針對(duì)該靶點(diǎn)的治療策略。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，適用于科研領(lǐng)域中對(duì)神經(jīng)系統(tǒng)疾病模型的機(jī)制研究、藥物干預(yù)效果評(píng)估或生物標(biāo)志物探索，例如多發(fā)性硬化癥、腦創(chuàng)傷或神經(jīng)退行性疾病的體外實(shí)驗(yàn)分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    MBP Ab
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, cerebrospinal fluid (CSF)
  • 檢測(cè)范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MBP antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 85-103  
    1:2 Average % 95  
    Range % 84-100  
    1:4 Average % 97  
    Range % 90-105  
    1:8 Average % 92  
    Range % 82-106  
  • 回收率：
    The recovery of human MBP antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 83-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.595 2.658 2.627 2.524  
    20 2.121 2.136 2.129 2.026  
    10 1.467 1.473 1.470 1.367  
    5 0.848 0.896 0.872 0.769  
    2.5 0.475 0.494 0.485 0.382  
    1.25 0.311 0.298 0.305 0.202  
    0.625 0.234 0.253 0.244 0.141  
    0 0.102 0.104 0.103 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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