Pig Procalcitonin,PCT ELISA Kit

產品詳情

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文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬降鈣素原(PCT)酶聯免疫試劑盒（CSB-E15004p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PCT含量。PCT（降鈣素原）是一種蛋白質。它健康狀態下在體內水平極低，感染時會顯著升高。其研究機制主要圍繞作為感染診斷和病情監測的生物標志物，在細菌感染時，機體產生炎癥反應促使PCT合成增加，可輔助判斷感染嚴重程度與預后。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，適用于科研場景中豬類疾病模型的炎癥進程監測、疫苗或藥物干預效果評估、免疫調控機制研究等方向，為獸醫科學、動物醫學研究及生物標志物開發提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PCT
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

39 pg/mL-2500 pg/mL
靈敏度：

9.75 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	95
	Range %	88-98
1:4	Average %	92
	Range %	89-96
1:8	Average %	93
	Range %	88-98

回收率：

The recovery of pig PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-96
EDTA plasma (n=4)	96	92-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.