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  4. Canine Procalcitonin(PCT)ELISA Kit

Canine Procalcitonin(PCT)ELISA Kit

  • 中文名稱：
    犬降鈣素原(PCT)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17400c
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 促銷(xiāo)：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬降鈣素原(PCT)酶聯(lián)免疫試劑盒（CSB-E17400c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液樣本中的PCT含量。PCT即降鈣素原，在健康人體中含量極低。細(xì)菌感染尤其是嚴(yán)重的全身感染時(shí)，PCT水平會(huì)顯著升高。其研究機(jī)制是細(xì)菌內(nèi)毒素等可刺激多種細(xì)胞產(chǎn)生PCT，臨床上常通過(guò)檢測(cè)其數(shù)值輔助判斷是否存在細(xì)菌感染、評(píng)估感染嚴(yán)重程度及預(yù)后。試劑盒檢測(cè)范圍為46.88 pg/mL-3000 pg/mL，在科研領(lǐng)域，該產(chǎn)品可用于探索犬類感染性疾病模型的病理機(jī)制、評(píng)估抗生素治療過(guò)程中炎癥反應(yīng)動(dòng)態(tài)變化，或作為免疫調(diào)節(jié)藥物開(kāi)發(fā)的生物標(biāo)志物檢測(cè)工具，為獸醫(yī)基礎(chǔ)研究及實(shí)驗(yàn)動(dòng)物科學(xué)提供可靠的定量分析解決方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    PCT
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測(cè)范圍：
    46.88 pg/mL-3000 pg/mL
  • 靈敏度：
    11.72 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 83-90
    1:2 Average % 95
    Range % 91-99
    1:4 Average % 91
    Range % 85-97
    1:8 Average % 83
    Range % 80-86
  • 回收率：
    The recovery of canine PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 84-93
    EDTA plasma (n=4) 104 101-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    3000 2.839 2.901 2.870 2.772
    1500 2.341 2.302 2.322 2.224
    750 1.602 1.657 1.630 1.532
    375 0.920 0.972 0.946 0.848
    187.5 0.425 0.408 0.417 0.319
    93.75 0.252 0.261 0.257 0.159
    46.88 0.144 0.152 0.148 0.050
    0 0.099 0.096 0.098 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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