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  4. Pig Angiopoietin 2,ANG-2 ELISA Kit

Pig Angiopoietin 2,ANG-2 ELISA Kit

  • 中文名稱：
    豬血管生成素2(ANG-2)酶聯免疫試劑盒
  • 貨號：
    CSB-E08460p
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    豬血管生成素2(ANG-2)酶聯免疫試劑盒（CSB-E08460p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ANGPT2含量。ANGPT2是一個備受關注的靶點。它在血管生成、血管穩態維持等方面發揮重要作用。其研究機制主要圍繞調控內皮細胞功能、影響血管通透性等。針對它的研究有望為腫瘤、心血管疾病等提供新的治療策略和藥物研發方向。試劑盒檢測范圍為0.94 ng/mL-60 ng/mL，適用于科研場景下豬模型的組織病理分析、血清/血漿循環標志物動態監測，或藥物干預后血管生成相關通路的機制研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    ANGPT2Angiopoietin-2 ELISA kit; ANG-2 ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.94 ng/mL-60 ng/mL
  • 靈敏度：
    0.235 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %86
    Range %83-89
    1:2Average %99
    Range %96-102
    1:4Average %87
    Range %84-90
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of pig ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-92
    EDTA plasma (n=4)9387-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    602.509 2.596 2.553 2.401
    301.749 1.768 1.759 1.607
    151.004 1.068 1.036 0.884
    7.50.663 0.642 0.653 0.501
    3.750.390 0.378 0.384 0.232
    1.880.298 0.290 0.294 0.142
    0.940.224 0.239 0.232 0.080
    00.151 0.153 0.152
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating angiogenic signals mediated by ANGPT1. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • 基因功能參考文獻：
    1. expression of angiopoietin 2 in the porcine metanephric kidney was observed in the podocytes of early developing glomeruli, but not in the cells near the glomerular hilus PMID: 20598355
    2. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. PMID: 20713984
  • 亞細胞定位：
    Secreted.
  • 數據庫鏈接：

    KEGG: ssc:396730

    STRING: 9823.ENSSSCP00000016694

    UniGene: Ssc.240