Mouse Hyaluronic acid,HA ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (11) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠透明質(zhì)酸(HA)酶聯(lián)免疫試劑盒（CSB-E08121m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HA含量。HA（血凝素）是流感病毒表面的糖蛋白，在病毒感染宿主細(xì)胞過程中起關(guān)鍵作用。其可與宿主細(xì)胞表面受體結(jié)合，介導(dǎo)病毒膜與細(xì)胞膜融合。研究HA有助于揭示流感病毒感染機(jī)制，為流感疫苗研發(fā)、抗病毒藥物設(shè)計提供重要靶點。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于科研領(lǐng)域?qū)A表達(dá)水平的動態(tài)監(jiān)測。在關(guān)節(jié)炎模型、皮膚再生研究、腫瘤微環(huán)境分析及代謝相關(guān)疾病機(jī)制探索中具有重要研究價值。適用于比較不同病理狀態(tài)下HA分泌水平差異或評估干預(yù)措施對HA代謝的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

HA
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-99
1:200	Average %	93
	Range %	89-101
1:400	Average %	102
	Range %	98-106
1:800	Average %	97
	Range %	92-102

回收率：

The recovery of mouse HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	97	93-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.