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  4. Human Hyaluronic acid,HA ELISA Kit

Human Hyaluronic acid,HA ELISA Kit

  • 中文名稱：
    人透明質酸(HA)酶聯免疫試劑盒
  • 貨號：
    CSB-E04805h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人透明質酸(HA)酶聯免疫試劑盒（CSB-E04805h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、腦脊液（CSF）、組織勻漿樣本中的HA含量。HA即血凝素，是流感病毒表面重要糖蛋白。其背景上，它在病毒感染宿主過程中至關重要。研究機制方面，HA可介導病毒與宿主細胞結合及膜融合，促使病毒侵入細胞。對HA研究有助于研發流感疫苗、抗病毒藥物等，以應對流感威脅。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，科研領域可應用于基礎醫學研究中HA代謝機制探索、藥物干預效果評價，或結合動物模型、細胞實驗進行跨物種、多組織類型的HA動態監測，為組織再生、炎癥調控及疾病機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    N/A
  • 縮寫：
    HA
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 87  
    Range % 80-94  
    1:40 Average % 92  
    Range % 88-99  
    1:80 Average % 97  
    Range % 91-100  
    1:160 Average % 101  
    Range % 97-105  
  • 回收率：
    The recovery of human HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 95-105  
    EDTA plasma (n=4) 93 88-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.727 2.700 2.714 2.626  
    5 2.231 2.345 2.288 2.200  
    2.5 1.558 1.655 1.607 1.519  
    1.25 0.831 0.901 0.866 0.778  
    0.625 0.477 0.451 0.464 0.376  
    0.312 0.301 0.322 0.312 0.224  
    0.156 0.189 0.174 0.182 0.094  
    0 0.087 0.089 0.088 　  
  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human HA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-labeled HA antibody(100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 2 個評價

樣品類型：細胞培養上清液

樣品信息：人

稀釋比：1:100

產品評價： 我用CSB-E04805h檢測血清樣本中HA含量，試劑盒靈敏度高，數據理想。

By 張老師

樣品類型：血漿（抗凝劑） edta

樣品信息：人

稀釋比：1:100

產品評價： 我們用CSB-E04805h檢測人冠心病患者血漿臨床樣本，檢測后的標曲擬合結果達到0.999，復孔的CV％變異系數＜10，效果較好，使用方便，為實驗提供了有力的支持

By 類老師

相關問答

■ 常見問題解答
Q:

We are looking into the best method to distinguish HA manufactured by fermentation and nature human HA.

A:
Thanks for your inquiry.
CSB-E04805h Human Hyaluronic acid, HA ELISA Kit
This kit can detect nature HA in polymerized form. As for HA manufactured by fermentation, it usually exists in free form.
We think it can not be detected out by the kit.

靶點詳情

  • 最新研究進展：
    HA（透明質酸）是一種大分子多糖，廣泛存在于人體組織中。最近的研究表明，HA在細胞間通訊、細胞遷移和組織再生中發揮重要作用。HA在軟骨組織再生和修復中的應用也受到廣泛關注。研究人員正在開發一些基于HA的藥物和材料，用于治療各種軟骨損傷和疾病，如骨關節炎、肩周炎和韌帶損傷等。