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  4. Human adrenocorticotropic hormone (ACTH) ELISA kit

Human adrenocorticotropic hormone (ACTH) ELISA kit

  • 中文名稱：
    人促腎上腺皮質激素(ACTH)酶聯免疫試劑盒
  • 貨號：
    CSB-E06873h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人促腎上腺皮質激素(ACTH)酶聯免疫試劑盒（CSB-E06873h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ACTH含量。ACTH即促腎上腺皮質激素，由腺垂體分泌。其背景在于它可促進腎上腺皮質增生、合成與分泌腎上腺皮質激素。研究機制方面，它與腎上腺皮質細胞膜上受體結合，激活腺苷酸環化酶-cAMP-蛋白激酶系統，從而調節腎上腺皮質激素合成與分泌。試劑盒檢測范圍為12.5 pg/mL-200 pg/mL，適用于科研領域中對下丘腦 - 垂體 - 腎上腺軸功能的機制研究、疾病模型構建以及藥物干預效果的評估；為研究ACTH在生理病理過程中的動態變化提供可靠工具，尤其適用于神經內分泌學、代謝性疾病及應激反應相關的基礎研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    ACTH
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    12.5 pg/mL-200 pg/mL
  • 靈敏度：
    12.5 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 82-93  
    1:2 Average % 94  
    Range % 89-99  
    1:4 Average % 95  
    Range % 89-99  
    1:8 Average % 92  
    Range % 87-96  
  • 回收率：
    The recovery of human ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 97 95-100  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.192 0.187 0.190    
    100 0.337 0.328 0.333    
    50 0.601 0.613 0.607    
    25 1.127 1.140 1.134    
    12.5 2.316 2.212 2.264    
  • 數據處理：
  • 貨期：
    3-5 working days

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