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Your Good Partner in Biology Research

Pig adrenocorticotropic hormone (ACTH) ELISA kit

  • 中文名稱:
    豬促腎上腺皮質激素(ACTH)酶聯免疫試劑盒
  • 貨號:
    CSB-E12944p
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    豬促腎上腺皮質激素(ACTH)酶聯免疫試劑盒(CSB-E12944p)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ACTH含量。促腎上腺皮質激素(ACTH)由腺垂體分泌,主要作用是促進腎上腺皮質增生、合成與分泌糖皮質激素。在研究機制上,它通過與腎上腺皮質細胞膜上受體結合,激活腺苷酸環化酶-cAMP-蛋白激酶系統發揮作用,對其研究有助于深入了解內分泌調控機制及相關疾病。試劑盒檢測范圍為6.25 pg/mL-100 pg/mL,該產品適用于動物生理機制研究、疾病模型內分泌功能評估或藥物干預實驗中的激素水平監測,例如探究應激條件下ACTH動態變化、腎上腺相關病理機制或藥效學評價等科研場景。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    ACTH
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    6.25 pg/mL-100 pg/mL
  • 靈敏度:
    6.25 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 86-96  
    1:2 Average % 99  
    Range % 92-104  
    1:4 Average % 84  
    Range % 80-87  
    1:8 Average % 91  
    Range % 83-99  
  • 回收率:
    The recovery of pig ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-99  
    EDTA plasma (n=4) 94 91-97  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    100 0.177 0.188 0.183    
    50 0.332 0.344 0.338    
    25 0.631 0.634 0.633    
    12.5 1.278 1.239 1.259    
    6.25 2.356 2.478 2.417    
  • 數據處理:
  • 貨期:
    3-5 working days

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