Dog interleukin 12 (IL-12/P70) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬白介素12(IL-12/P70)酶聯(lián)免疫試劑盒（CSB-EQ027628DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL12/P70含量。IL12/P70是一種重要的靶點。它在免疫調(diào)節(jié)中起關(guān)鍵作用，能促進(jìn)Th1細(xì)胞分化、增強(qiáng)NK細(xì)胞活性等。研究主要圍繞其參與的免疫信號通路，探索通過調(diào)控它來治療如自身免疫性疾病、腫瘤等，有望為相關(guān)疾病治療帶來新策略。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于犬類免疫機(jī)制研究，包括炎癥性疾病模型建立、疫苗免疫效果評估或感染后細(xì)胞因子動態(tài)監(jiān)測等科研領(lǐng)域，可為動物免疫學(xué)、傳染病學(xué)及比較醫(yī)學(xué)研究提供可靠的實驗數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL12/P70, Interleukin-12/P70
縮寫：

IL-12/P70
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-105
1:2	Average %	87
	Range %	84-93
1:4	Average %	97
	Range %	91-106
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of dog IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	97	92-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.