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  4. Pig Interleukin 12,IL-12/P70 ELISA Kit

Pig Interleukin 12,IL-12/P70 ELISA Kit

  • 中文名稱：
    豬白介素12(IL-12/P70)酶聯免疫試劑盒
  • 貨號：
    CSB-E13288p
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    豬白介素12(IL-12/P70)酶聯免疫試劑盒（CSB-E13288p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL12/P70含量。IL12/P70是一種重要的靶點。它在免疫系統中發揮關鍵作用，能夠調節免疫細胞功能，參與機體炎癥反應和免疫應答過程。相關研究主要聚焦于其信號傳導通路和與疾病的關聯機制，有望為炎癥性疾病、腫瘤等的治療提供新策略。試劑盒檢測范圍為0.45 ng/mL-30 ng/mL，靈敏度為0.11 ng/mL。適用于豬類動物模型中的免疫調控機制研究、疫苗效力評估、感染性疾病或炎癥反應模型中細胞因子網絡的動態分析；適用于畜牧獸醫研究、免疫毒理學實驗及生物制劑開發等領域的定量分析需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IL12A ELISA Kit; Interleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
  • 縮寫：
    IL-12/P70
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.45 ng/mL-30 ng/mL
  • 靈敏度：
    0.11 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %96
    Range %91-101
    1:4Average %84
    Range %80-89
    1:8Average %93
    Range %88-97
  • 回收率：
    The recovery of pig IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-95
    EDTA plasma (n=4)9490-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    302.549 2.452 2.501 2.331
    151.789 1.761 1.775 1.605
    7.51.025 1.004 1.015 0.845
    3.750.591 0.549 0.570 0.400
    1.80.402 0.374 0.388 0.218
    0.90.321 0.309 0.315 0.145
    0.450.258 0.245 0.252 0.082
    00.173 0.167 0.170 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 數據庫鏈接：

    KEGG: ssc:397053

    STRING: 9823.ENSSSCP00000012501

    UniGene: Ssc.13