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LGR5

LGR5(富含亮氨酸重復(fù)序列的G蛋白偶聯(lián)受體5)是Wnt信號通路的關(guān)鍵受體,又稱G蛋白偶聯(lián)受體49(GPR49),在腸道、毛囊等組織的干細(xì)胞中特異性表達(dá),被視為成體干細(xì)胞的標(biāo)志物。LGR5通過與R-spondin家族蛋白結(jié)合,增強(qiáng)β-連環(huán)蛋白的穩(wěn)定性,調(diào)控干細(xì)胞自我更新與組織再生。LGR5在結(jié)直腸癌、胃癌等惡性腫瘤中高表達(dá),其陽性細(xì)胞群體常表現(xiàn)出腫瘤干細(xì)胞特性,與化療耐藥、復(fù)發(fā)轉(zhuǎn)移密切相關(guān)。近年來針對LGR5的藥物開發(fā)聚焦于單克隆抗體和小分子抑制劑,其中LGR5-ADC(抗體藥物偶聯(lián)物)在臨床前研究中顯示出靶向清除腫瘤干細(xì)胞的潛力。基于LGR5陽性細(xì)胞的CAR-T療法也在探索中,但需克服靶向正常干細(xì)胞的脫靶風(fēng)險。在再生醫(yī)學(xué)領(lǐng)域,調(diào)控LGR5信號通路為潰瘍性結(jié)腸炎、放射性腸損傷等疾病提供了新思路。

熱銷產(chǎn)品

Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5)-VLPs (Active) (CSB-MP012906HU(A4))

驗(yàn)證數(shù)據(jù)

CSB-MP012906HU(A4)

CSB-MP012906HU(A4) is detected by Mouse anti-10*His monoclonal antibody.

CSB-MP012906HU(A4)

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human LGR5 at 10 μg/ml can bind Anti-LGR5 recombinant antibody (CSB-RA012906MA1HU). The EC50 is 1.690-1.867 ng/mL.The VLPs (CSB-MP3838) is negative control.

LGR5 Recombinant Monoclonal Antibody (CSB-RA262034A0HU)

驗(yàn)證數(shù)據(jù)

CSB-RA262034A0HU

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: LGR5 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 100, 98, 93 kDa
Observed band size: 100 kDa

CSB-RA262034A0HU

Overlay histogram showing HepG2 cells stained with CSB-RA262034A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.

LGR5 Antibody (CSB-PA012906LA01HU)

驗(yàn)證數(shù)據(jù)

CSB-PA012906LA01HU

IHC image of CSB-PA012906LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-PA012906LA01HU

Immunofluorescence staining of HepG2 cells with CSB-PA012906LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

LGR5 Antibodies

LGR5 for Homo sapiens (Human)