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  4. Recombinant Drosophila melanogaster Proliferating cell nuclear antigen (mus209)

Recombinant Drosophila melanogaster Proliferating cell nuclear antigen (mus209)

  • 中文名稱：
    黑腹果蠅PCNA重組蛋白
  • 貨號：
    CSB-YP017621DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅PCNA重組蛋白
  • 貨號：
    CSB-EP017621DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅PCNA重組蛋白
  • 貨號：
    CSB-EP017621DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅PCNA重組蛋白
  • 貨號：
    CSB-BP017621DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅PCNA重組蛋白
  • 貨號：
    CSB-MP017621DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    PCNA; mus209; PCNA1; CG9193; Proliferating cell nuclear antigen; PCNA; Cyclin; Mutagen-sensitive 209 protein
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-260
  • 氨基酸序列
    MFEARLGQAT ILKKILDAIK DLLNEATFDC SDSGIQLQAM DNSHVSLVSL TLRSDGFDKF RCDRNLSMGM NLGSMAKILK CANNEDNVTM KAQDNADTVT IMFESANQEK VSDYEMKLMN LDQEHLGIPE TDFSCVVRMP AMEFARICRD LAQFSESVVI CCTKEGVKFS ASGDVGTANI KLAQTGSVDK EEEAVIIEMQ EPVTLTFACR YLNAFTKATP LSTQVQLSMC ADVPLVVEYA IKDLGHIRYY LAPKIEDNET
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Likely to be an auxiliary protein of DNA polymerase delta complex and is probably involved in the control of DNA replication and repair by increasing the polymerase's processibility.
  • 基因功能參考文獻：
    1. crystal of DmPCNA diffracted to 2.0 A resolution and belonged to space group H3, with unit-cell parameters a = b = 151.16, c = 38.28 A PMID: 23545643
    2. We observed that SUUR chromosomal localization changed along with PCNA pattern and these proteins largely co-localized during the late S-phase in salivary glands. PMID: 23149855
    3. E2F regulation of PCNA is dispensable for viability, but is nonetheless important for normal Drosophila development. PMID: 12526745
    4. A Q123V mutation dramaticly enhances the abilities of Drosophila PCNA to stimulate calf thymus pol delta. Replacing the entire interdomain connector loop AA 119-133 with the corresponding residues from human PCNA results in additional enhancement. PMID: 15310391
  • 亞細胞定位：
    Nucleus. Chromosome. Cytoplasm.
  • 蛋白家族：
    PCNA family
  • 組織特異性：
    Expressed at high levels in adult ovary.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG9193

    STRING: 7227.FBpp0085619

    UniGene: Dm.4751