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  4. Rat secretory immunoglobulin A,sIgA ELISA Kit

Rat secretory immunoglobulin A,sIgA ELISA Kit

  • 中文名稱：
    大鼠分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08412r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒（CSB-E08412r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的sIgA含量。sIgA即分泌型免疫球蛋白A，是黏膜免疫的重要效應(yīng)分子。它可阻止病原體黏附上皮細胞、中和毒素及病毒等。研究主要聚焦其產(chǎn)生機制、在黏膜免疫防御中的作用機制等，對揭示機體抗感染免疫及開發(fā)相關(guān)疫苗等有重要意義。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，在科研領(lǐng)域，本試劑盒可用于研究大鼠模型中黏膜免疫相關(guān)疾病（如腸道炎癥、呼吸道感染）的發(fā)病機制，評估免疫調(diào)節(jié)劑或益生菌干預(yù)效果，亦可應(yīng)用于營養(yǎng)學、毒理學研究中黏膜屏障功能的動態(tài)監(jiān)測。其多樣本類型兼容性為實驗設(shè)計提供靈活性，例如通過組織勻漿檢測局部黏膜sIgA表達，或通過體液樣本評估系統(tǒng)免疫狀態(tài)。本產(chǎn)品嚴格遵循質(zhì)量控制標準，提供標準品及預(yù)包被板等完整實驗組件，確保實驗數(shù)據(jù)的穩(wěn)定性和可重復(fù)性，為科研人員探索黏膜免疫調(diào)控網(wǎng)絡(luò)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    sIgA
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %102
    Range %97-106
    1:200Average %90
    Range %86-96
    1:400Average %97
    Range %92-103
    1:800Average %88
    Range %84-92
  • 回收率：
    The recovery of rat sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8480-88
    EDTA plasma (n=4)9591-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.511 2.371 2.441 2.302
    252.046 1.976 2.011 1.872
    12.51.512 1.501 1.507 1.368
    6.250.979 1.003 0.991 0.852
    3.120.482 0.518 0.500 0.361
    1.560.334 0.359 0.347 0.208
    0.780.241 0.229 0.235 0.096
    00.143 0.135 0.139 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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