Rat prothrombin fragment 1+2,F1+2 ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠凝血酶原片段F1+2(F1+2)酶聯免疫試劑盒（CSB-E13264r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的F1+2含量。試劑盒檢測范圍為62.5 pmol/L-4000 pmol/L，適用于研究凝血與纖溶系統失衡相關疾病機制，如血栓形成模型、抗凝藥物藥效評價或血管內皮損傷實驗中的凝血激活狀態監測。科研人員可通過本試劑盒精準量化大鼠模型在病理生理條件下凝血酶原活化水平，為心血管疾病、彌散性血管內凝血（DIC）等研究提供可靠數據支持，但不可用于臨床診斷或治療監測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

F1+2
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pmol/L-4000 pmol/L
靈敏度：

15.6 pmol/L
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	102
	Range %	92-110
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rat F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.