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Rat activated coagulation factor X(FXa) ELISA Kit

說明書下載

產品詳情

產品描述：

大鼠活化凝血因子X(FXa)酶聯免疫試劑盒（CSB-EQ027251RA）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FXa含量。FXa即凝血因子Xa，是凝血級聯反應中的關鍵靶點。它在凝血過程中處于內、外源凝血途徑交匯點，激活后促使凝血酶原轉化為凝血酶。對其研究機制主要聚焦于開發抑制劑，以抑制凝血過程，用于血栓性疾病防治。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，支持實驗室開展凝血機制研究、抗凝藥物篩選、病理模型評估等方向實驗，多樣本兼容性設計尤其適合系統性研究凝血因子在組織微環境中的動態變化，為探索血栓性疾病、術后凝血異常等課題提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

FXa
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	102
	Range %	98-109
1:400	Average %	90
	Range %	86-94
1:800	Average %	97
	Range %	92-101

回收率：

The recovery of rat FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	85-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.