Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠基質細胞衍生因子1a(SDF-1a/CXCL12)酶聯免疫試劑盒（CSB-E08729r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的SDF1a/CXCL12含量。SDF1a/CXCL12是重要的趨化因子。其背景上，在胚胎發育、免疫調節等生理過程發揮關鍵作用。研究機制方面，與受體CXCR4結合，介導細胞遷移、增殖和存活，在腫瘤轉移、炎癥等病理進程中有重要影響，是相關疾病治療潛在靶點。試劑盒檢測范圍為0.78 pg/mL-50 pg/mL，可廣泛應用于神經再生、造血調控、免疫細胞趨化性等基礎研究領域，為探索SDF - 1a/CXCL12在疾病模型中的動態變化提供高特異性檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

SDF1a/CXCL12
Uniprot No.：

Q9QZD1
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.78 pg/mL-50 pg/mL
靈敏度：

0.195 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SDF-1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	83-97
1:200	Average %	97
	Range %	91-103
1:400	Average %	84
	Range %	80-88
1:800	Average %	89
	Range %	81-95

回收率：

The recovery of rat SDF-1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-96
EDTA plasma (n=4)	97	94-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.