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Your Good Partner in Biology Research

Rat Myeloperoxidase (MPO) ELISA kit

  • 中文名稱:
    大鼠髓過氧化物酶(MPO)酶聯免疫試劑盒
  • 貨號:
    CSB-E08722r
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠髓過氧化物酶(MPO)酶聯免疫試劑盒(CSB-E08722r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的MPO含量。MPO(髓過氧化物酶)是一種血紅素蛋白酶,存在于中性粒細胞中,參與免疫和炎癥反應。其研究背景涉及其在多種疾病中的作用,如心血管疾病和腫瘤。研究機制主要關注其基因表達、酶活性及其與宿主防御的相互作用。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,該產品廣泛應用于炎癥機制研究、動脈粥樣硬化模型評估、神經退行性疾病研究等科研領域,也可用于藥物干預實驗中生物標志物的動態監測。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %94
    Range %90-98
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-102
  • 回收率:
    The recovery of rat MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9287-98
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.347 2.232 2.290 2.109
    101.416 1.386 1.401 1.220
    50.902 0.887 0.895 0.714
    2.50.602 0.612 0.607 0.426
    1.250.421 0.439 0.430 0.249
    0.6250.312 0.326 0.319 0.138
    0.3120.274 0.256 0.265 0.084
    00.182 0.180 0.181  
  • 數據處理:
  • 貨期:
    3-5 working days

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