Rat Immunoglobulin M,IgM ELISA Kit

產品詳情

文獻引用 (10) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠免疫球蛋白M(IgM)酶聯免疫試劑盒（CSB-E07978r）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgM含量。IgM是人體免疫系統中重要的免疫球蛋白，在早期免疫防御里發揮關鍵作用。其在自身免疫病、感染性疾病等的發生發展中意義重大。研究機制方面，聚焦其與抗原結合、激活補體系統等特性，探索以其為靶點開發診斷方法和治療藥物。試劑盒檢測范圍為6.25 ng/mL-1600 ng/mL，適用于科研領域對大鼠體液免疫狀態的評估，例如感染性疾病模型中病原體特異性抗體的動態監測、疫苗研發過程中免疫原性分析，或自身免疫疾病研究中的抗體水平追蹤。適用于基礎免疫學、病理機制研究及生物制劑開發等場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgM
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

6.25 ng/mL-1600 ng/mL
靈敏度：

6.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	95-104
1:2000	Average %	90
	Range %	86-95
1:4000	Average %	89
	Range %	83-93
1:8000	Average %	100
	Range %	95-105

回收率：

The recovery of rat IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	92-97
EDTA plasma (n=4)	93	89-97