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  4. Rat Immunoglobulin E,IgE ELISA Kit

Rat Immunoglobulin E,IgE ELISA Kit

  • 中文名稱：
    大鼠免疫球蛋白E(IgE)酶聯免疫試劑盒
  • 貨號：
    CSB-E07984r
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠免疫球蛋白E(IgE)酶聯免疫試劑盒（CSB-E07984r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IgE含量。IgE即免疫球蛋白 E，是參與過敏性疾病發生發展的重要靶點。其背景在于它與速發型過敏反應密切相關。研究機制主要是，IgE 可與肥大細胞、嗜堿性粒細胞表面受體結合，當變應原再次入侵，引發 IgE 交聯，促使細胞釋放炎性介質，從而導致過敏癥狀。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，適用于過敏機制研究、寄生蟲感染模型評估、免疫調節藥物篩選等科研領域，為探索Th2型免疫反應、肥大細胞激活等研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    IgE
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %84-93
    1:2Average %96
    Range %91-103
    1:4Average %84
    Range %81-89
    1:8Average %93
    Range %89-97
  • 回收率：
    The recovery of rat IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-98
    EDTA plasma (n=4)9590-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.055 2.086 2.071 1.926
    1001.520 1.649 1.585 1.440
    501.101 1.156 1.129 0.984
    250.738 0.750 0.744 0.599
    12.50.417 0.445 0.431 0.286
    6.250.341 0.337 0.339 0.194
    3.120.201 0.213 0.207 0.062
    00.139 0.150 0.145 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat IgE antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled IgE antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

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