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  4. Rat Homocysteine(Hcy)ELISA Kit

Rat Homocysteine(Hcy)ELISA Kit

  • 中文名稱：
    大鼠高半胱氨酸(Hcy)酶聯免疫試劑盒
  • 貨號：
    CSB-E13376r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠高半胱氨酸(Hcy)酶聯免疫試劑盒（CSB-E13376r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Hcy含量。Hcy即同型半胱氨酸，是蛋氨酸代謝的中間產物。其水平升高是心腦血管疾病等多種疾病的獨立危險因素。研究機制上，可能通過損傷血管內皮細胞、促進血栓形成、氧化應激等途徑參與疾病發生發展，對其深入研究有重要臨床意義。試劑盒檢測范圍為0.78 nmol/mL-50 nmol/mL，廣泛應用于大鼠模型的基礎研究，例如在高血壓、動脈粥樣硬化或阿爾茨海默病等動物模型中評估Hcy水平變化，或用于探究營養干預、藥物代謝對Hcy調控的影響，為疾病機制解析及治療策略開發提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    N/A
  • 縮寫：
    Hcy
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.78 nmol/mL-50 nmol/mL
  • 靈敏度：
    0.195 nmol/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %87-95
    1:2Average %94
    Range %90-98
    1:4Average %94
    Range %89-97
    1:8Average %100
    Range %93-103
  • 回收率：
    The recovery of rat Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-97
    EDTA plasma (n=4)9592-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/mlOD1OD2AverageCorrected
    502.874 2.771 2.823 2.670
    252.223 2.245 2.234 2.081
    12.51.418 1.389 1.404 1.251
    6.250.756 0.775 0.766 0.613
    3.120.462 0.448 0.455 0.302
    1.560.321 0.313 0.317 0.164
    0.780.232 0.225 0.229 0.076
    00.155 0.151 0.153 　
  • 數據處理：
  • 貨期：
    3-5 working days

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