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Your Good Partner in Biology Research

Rat High density lipoprotein cholesterol, HDL-C ELISA kit

  • 中文名稱:
    大鼠高密度脂蛋白膽固醇酶聯免疫試劑盒
  • 貨號:
    CSB-E14399r
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠高密度脂蛋白膽固醇酶聯免疫試劑盒(CSB-E14399r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的HDL-C含量。HDL - C即高密度脂蛋白膽固醇,它被視作“好膽固醇”,背景上與心血管健康密切相關,較高水平的HDL - C有助于降低心血管疾病風險。其研究機制主要是促進膽固醇逆向轉運,將外周組織中的膽固醇轉運到肝臟進行代謝,減少膽固醇沉積。試劑盒檢測范圍為93.75 ng/mL-6000 ng/mL,適用于動脈粥樣硬化發病機制研究、脂代謝調控實驗、藥物干預效果評價等科研領域,可支持動物模型構建、營養學實驗及心血管相關分子通路的探索。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    HDL-C
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    93.75 ng/mL-6000 ng/mL
  • 靈敏度:
    23.4 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %87
    Range %83-89
    1:400Average %99
    Range %95-102
    1:800Average %90
    Range %85-93
    1:1600Average %99
    Range %92-102
  • 回收率:
    The recovery of rat HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)107101-109
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    60002.612 2.554 2.583 2.483
    30002.122 2.158 2.140 2.040
    15001.449 1.453 1.451 1.351
    7500.845 0.831 0.838 0.738
    3750.420 0.434 0.427 0.327
    187.50.272 0.278 0.275 0.175
    93.750.176 0.169 0.173 0.073
    00.101 0.099 0.100
  • 數據處理:
  • 貨期:
    3-5 working days

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