Rat Calcitonin,CT ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述:大鼠降鈣素(CT)酶聯(lián)免疫試劑盒(CSB-E05132r)檢測(cè)血清、血漿、組織勻漿樣本中的CALCA含量。CALCA 是重要靶點(diǎn)。它在人體生理過(guò)程有諸多參與。對(duì)其研究機(jī)制主要從基因表達(dá)調(diào)控等方面探究,目的是明確它在疾病發(fā)生發(fā)展中的作用,為開(kāi)發(fā)針對(duì)該靶點(diǎn)的藥物、開(kāi)展精準(zhǔn)治療等奠定基礎(chǔ),有望推動(dòng)相關(guān)疾病治療的進(jìn)步。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL,大鼠模型中的鈣代謝調(diào)控機(jī)制探索、骨骼疾病動(dòng)物模型評(píng)估、甲狀腺相關(guān)腫瘤分子機(jī)制研究等實(shí)驗(yàn)場(chǎng)景本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:CALCA Calcitonin
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縮寫(xiě):
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:1.56 pg/mL-100 pg/mL
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靈敏度:0.39 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 87-101 1:2 Average % 88 Range % 80-95 1:4 Average % 95 Range % 86-106 1:8 Average % 90 Range % 82-100 -
回收率:
The recovery of rat CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 85-99 EDTA plasma (n=4) 94 88-101 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 100 2.746 2.779 2.763 2.582 50 2.138 2.175 2.157 1.976 25 1.395 1.453 1.424 1.243 12.5 0.866 0.854 0.860 0.679 6.25 0.552 0.562 0.557 0.376 3.12 0.428 0.401 0.415 0.234 1.56 0.283 0.298 0.291 0.110 0 0.179 0.183 0.181 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- PER1 Oscillation in Rat Parathyroid Hormone and Calcitonin Producing Cells B Georg, HL J?rgensen, J Hannibal,International Journal of Molecular Sciences,2024
- The crucial role of beta-catenin in the osteoprotective effect of semaglutide in an ovariectomized rat model of osteoporosis MHH Abo-Elenin, R Kamel, S Nofal,Naunyn-Schmiedeberg's Archives of Pharmacology,2024
- Bone Developmental Toxicology Studies of Cadmium Chloride in Juvenile Sprague Dawley Rats S Wang,/,2024
- Correlation between Antioxidant and Anti-Osteoporotic Activities of Shilajit Loaded into Chitosan Nanoparticles and Their Effects on Osteoporosis in Rats FA Alshubaily,Polymers,2022
- Metallothionein deficiency aggravates depleted uranium-induced nephrotoxicity Hao Y. et al,Toxicol Appl Pharmacol. ,2015
相關(guān)問(wèn)答
我需要一種高靈敏度的降鈣素ELISA試劑盒,因?yàn)槲疑洗问褂玫脑噭┖徐`敏度不夠,不能檢測(cè)血漿中的降鈣素水平。你們能否保證可以將降鈣素的檢測(cè)范圍限制在2 pg/ml以下?
靶點(diǎn)詳情
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功能:Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
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亞細(xì)胞定位:Secreted.
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蛋白家族:Calcitonin family
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數(shù)據(jù)庫(kù)鏈接:
STRING: 10116.ENSRNOP00000014948
UniGene: Rn.90085
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