Rat β2-microglobulin,BMG/β2-MG ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠β2微球蛋白(BMG/β2-MG)酶聯(lián)免疫試劑盒（CSB-E11298r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、尿液、組織勻漿樣本中的B2M含量。B2M即β2-微球蛋白，是MHCⅠ類(lèi)分子的輕鏈部分，廣泛存在于有核細(xì)胞表面。其在免疫識(shí)別、抗原呈遞中發(fā)揮重要作用。研究機(jī)制圍繞免疫調(diào)節(jié)展開(kāi)，如它影響T細(xì)胞對(duì)靶細(xì)胞的識(shí)別。此外，其表達(dá)異常與多種疾病如腫瘤、自身免疫病相關(guān)。試劑盒檢測(cè)范圍為0.156 μg/mL-10 μg/mL，適用于探索腎臟生理病理機(jī)制、評(píng)估藥物腎毒性、腫瘤免疫微環(huán)境分析等基礎(chǔ)研究領(lǐng)域，尤其為尿液樣本的腎小管功能研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

B2MBeta-2-microglobulin ELISA kit
縮寫(xiě)：

B2M
Uniprot No.：

P01885
種屬：

Rattus norvegicus (Rat)
樣本類(lèi)型：

serum, plasma, urine, tissue homogenates
檢測(cè)范圍：

0.156 μg/mL-10 μg/mL
靈敏度：

0.039 μg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat BMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	96
	Range %	92-101
1:4	Average %	100
	Range %	95-105
1:8	Average %	90
	Range %	85-95

回收率：

The recovery of rat BMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	93	89-97

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Beta-2-microglobulin family
數(shù)據(jù)庫(kù)鏈接：

STRING: 9986.ENSOCUP00000014707