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  4. Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit

Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit

  • 中文名稱：
    兔子基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14220Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒（CSB-E14220Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP13含量。MMP13即基質(zhì)金屬蛋白酶13，是參與細(xì)胞外基質(zhì)代謝的關(guān)鍵酶。其異常表達(dá)與多種疾病的發(fā)生發(fā)展密切相關(guān)，如骨關(guān)節(jié)炎、腫瘤轉(zhuǎn)移等。研究主要圍繞其在疾病進(jìn)程中的作用機(jī)制，探索以它為靶點(diǎn)開發(fā)疾病治療藥物的可能。試劑盒檢測范圍為93.75 pg/mL-6000 pg/mL，本試劑盒適用于體外實(shí)驗(yàn)研究，可滿足關(guān)節(jié)炎模型構(gòu)建、骨代謝研究、腫瘤微環(huán)境分析等科研場景中MMP - 13的動(dòng)態(tài)監(jiān)測需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    MMP13Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    93.75 pg/mL-6000 pg/mL
  • 靈敏度：
    23.44 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %105
    Range %97-108
    1:4Average %90
    Range %85-95
    1:8Average %95
    Range %89-100
  • 回收率：
    The recovery of rabbit MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9083-95
    EDTA plasma (n=4)9890-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    60002.554 2.444 2.499 2.405
    30001.937 1.976 1.957 1.863
    15001.409 1.422 1.416 1.322
    7500.815 0.789 0.802 0.708
    3750.422 0.434 0.428 0.334
    187.50.302 0.289 0.296 0.202
    93.750.207 0.198 0.203 0.109
    00.093 0.095 0.094
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
  • 基因功能參考文獻(xiàn)：
    1. These results suggest that PEP-1-SIRT2 promotes matrix metalloproteinases-induced dedifferentiation via ERK signaling in articular chondrocytes. PMID: 27697532
    2. the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID: 23879595
    3. chitosan-pDNA nanoparticles encoding shRNA targeting MMP-3 and -13 had great potential in silencing the dedifferentiation-related genes for regenerating prolonged and endurable cartilage. PMID: 23520014
    4. Leptin can induce MMP-13 and have a synergistic induction effect on NO with TNF-alpha in rabbit articular chondrocytes in vitro. PMID: 19893248
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Peptidase M10A family
  • 數(shù)據(jù)庫鏈接：