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Pig Tri-iodothyronine,T3 ELISA Kit

文獻引用 (1) 說明書下載

產品詳情

產品描述：

豬三碘甲狀腺原氨酸(T3)酶聯免疫試劑盒（CSB-E14195p）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的T3含量。豬三碘甲狀腺原氨酸（T3）是甲狀腺分泌的激素，由L-3-單碘酪氨酸與L-3.5-二碘酪氨酸縮合而成，具有促進物質代謝和能量釋放的作用，對生長與發育也有促進作用。其活性比甲狀腺素（T4）高5～10倍，主要通過促進T4在外周轉化為T3來發揮作用。試劑盒檢測范圍為500 pg/mL-8000 pg/mL，靈敏度為500 pg/mL。適用于動物內分泌生理機制探索、營養代謝實驗或疾病模型中激素水平變化的動態監測，其寬泛的檢測范圍可有效覆蓋正常生理及病理狀態下的T3濃度波動，為科研人員提供穩定可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

T3
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

500 pg/mL-8000 pg/mL
靈敏度：

500 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pigT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	98
	Range %	87-105
1:4	Average %	92
	Range %	85-100
1:8	Average %	95
	Range %	90-102

回收率：

The recovery of pig T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	97	93-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進展：

T3作為一種內源性甲狀腺激素，最近的研究發現它與糖代謝、骨骼生長發育、血壓控制等方面密切相關。另外，T3還被證實能夠干預神經系統和免疫系統等生理過程，這些研究為T3的臨床應用提供了新的思路和方向。