Pig Neuron-specific enolase,NSE ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬神經(jīng)特異性烯醇化酶(NSE)酶聯(lián)免疫試劑盒（CSB-E14065p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的NSE含量。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，適用于腦組織、外周神經(jīng)等樣本的神經(jīng)功能評(píng)估，兼容常規(guī)血清/血漿樣本（需離心去除沉淀后分裝凍存），可滿足神經(jīng)退行性疾病模型構(gòu)建、神經(jīng)損傷修復(fù)研究以及神經(jīng)內(nèi)分泌相關(guān)疾病機(jī)制探索等科研需求，為動(dòng)物實(shí)驗(yàn)中的病理機(jī)制解析和藥物干預(yù)效果觀察提供可靠定量工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

NSE
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-102
1:2	Average %	90
	Range %	85-99
1:4	Average %	95
	Range %	87-98
1:8	Average %	94
	Range %	86-104

回收率：

The recovery of pig NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	84-102
EDTA plasma (n=4)	94	83-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.