Pig Collagen Type Ⅲ,Col Ⅲ ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬Ⅲ型膠原(Col Ⅲ)酶聯(lián)免疫試劑盒（CSB-E06776p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅲ含量。Col Ⅲ（膠原蛋白Ⅲ）是細(xì)胞外基質(zhì)的主要成分之一，參與維持肺動脈管壁的彈性和完整性。在肺動脈高壓（PAH）等疾病中，TGF-β1等因子促進(jìn)PASMCs外膠原沉積，導(dǎo)致肺血管重構(gòu)。低濃度紫杉醇（PTX）可通過抑制TGF-β1/Smad3信號通路，降低COL Ⅲ表達(dá)，從而逆轉(zhuǎn)纖維化進(jìn)程。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于探究豬模型在纖維化機(jī)制、心血管疾病或皮膚再生等領(lǐng)域的科研需求，為組織工程、病理模型構(gòu)建及分子機(jī)制研究提供可靠數(shù)據(jù)支持，是基礎(chǔ)研究與轉(zhuǎn)化醫(yī)學(xué)中膠原代謝相關(guān)課題的理想工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
縮寫：

Col Ⅲ
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	91-103
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	93
	Range %	89-95

回收率：

The recovery of pig Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	92-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.