Pig C-Peptide ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬C肽(C-Peptide)酶聯(lián)免疫試劑盒（CSB-E15977p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C-Peptide含量。C-Peptide即C肽，是胰島素原裂解產(chǎn)生的連接肽。其背景在于可反映胰島β細(xì)胞功能，不受外源性胰島素影響。研究機(jī)制方面，主要圍繞評估胰島素分泌、輔助糖尿病分型等，還在探索其對改善神經(jīng)、血管病變等的潛在作用。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，適用于實(shí)驗(yàn)室條件下動(dòng)態(tài)監(jiān)測豬胰島功能狀態(tài)、評估糖尿病動(dòng)物模型干預(yù)效果或探究代謝性疾病相關(guān)分子機(jī)制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

C-Peptide
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.25 ng/mL-80 ng/mL
靈敏度：

0.312 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-98
1:2	Average %	96
	Range %	91-102
1:4	Average %	103
	Range %	97-107
1:8	Average %	87
	Range %	81-93

回收率：

The recovery of pig C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-104
EDTA plasma (n=4)	92	87-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.