Mouse advanced glycation end products,AGEs ELISA Kit

產品詳情

文獻引用 (10) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠晚期糖基化終末產物(AGEs)酶聯免疫試劑盒（CSB-E09414m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AGEs含量。AGEs是晚期糖基化終末產物。背景上，它是糖與蛋白質等非酶促反應的產物，在糖尿病、衰老等過程中積累。研究機制表明，其通過與受體結合，激活多條信號通路，引發氧化應激、炎癥反應等，參與糖尿病并發癥等多種疾病發生發展。試劑盒檢測范圍為0.156 μg/mL-10 μg/mL，科研人員可借助探究AGEs在代謝性疾病模型中的動態變化、評估抗氧化或抗糖化藥物的干預效果，以及衰老相關分子機制研究，為生物醫學領域提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

AGEs
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 μg/mL-10 μg/mL
靈敏度：

0.039 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	97
	Range %	92-103
1:4	Average %	99
	Range %	94-110
1:8	Average %	95
	Range %	87-100

回收率：

The recovery of mouse AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	84-99
EDTA plasma (n=4)	97	92-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














µg/ml	OD1	OD2	Average	Corrected
10	1.958	1.900	1.929	1.779
5	1.583	1.602	1.593	1.443
2.5	1.160	1.154	1.157	1.007
1.25	0.754	0.739	0.747	0.597
0.625	0.471	0.434	0.453	0.303
0.312	0.342	0.319	0.331	0.181
0.156	0.253	0.239	0.246	0.096
0	0.152	0.148	0.150