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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Mouse Interleukin 23,IL-23 ELISA Kit
  • 中文名稱(chēng)：
    小鼠白介素23(IL-23)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08463m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 促銷(xiāo)：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠白介素23(IL-23)酶聯(lián)免疫試劑盒（CSB-E08463m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL-23含量。IL-23是一種細(xì)胞因子，在免疫系統(tǒng)中發(fā)揮重要作用。它能夠調(diào)節(jié)Th17細(xì)胞的生成與功能，與自身免疫疾病、炎癥反應(yīng)密切相關(guān)。目前針對(duì)IL-23的研究聚焦于其信號(hào)通路及作用機(jī)制，以開(kāi)發(fā)相關(guān)治療藥物來(lái)改善炎癥和免疫異常狀況。試劑盒檢測(cè)范圍為7.8 pg/ml-500 pg/ml，適用于科研領(lǐng)域中對(duì)感染性疾病模型、炎癥反應(yīng)機(jī)制、免疫調(diào)節(jié)研究的IL - 23動(dòng)態(tài)監(jiān)測(cè)，也可用于評(píng)估藥物或生物制劑對(duì)IL - 23通路的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    N/A
  • 縮寫(xiě)：
    IL-23
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    7.8 pg/ml-500 pg/ml
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 88    
    Range % 81-92    
    1:2 Average % 95    
    Range % 89-100    
    1:4 Average % 93    
    Range % 84-97    
    1:8 Average % 106    
    Range % 97-110    
  • 回收率：
    The recovery of mouse IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 104 94-108    
    EDTA plasma (n=4) 101 94-104    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    500 2.868 2.689 2.779 2.654    
    250 1.519 1.586 1.553 1.428    
    125 0.746 0.700 0.723 0.598    
    62.5 0.434 0.456 0.445 0.320    
    31.2 0.314 0.301 0.308 0.183    
    15.6 0.207 0.202 0.205 0.080    
    7.8 0.153 0.159 0.156 0.031    
    0 0.127 0.123 0.125 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類(lèi)型：組織勻漿液 海馬組織

樣品信息：小鼠

稀釋比：1:50

產(chǎn)品評(píng)價(jià)： 我用CSB-E08463m分別檢測(cè)了磁刺激前后小鼠海馬組織勻漿中IL-23水平，標(biāo)曲結(jié)果良好，發(fā)現(xiàn)BTBR小鼠IL-23水平高于C57小鼠，磁刺激后IL-23水平降低，與預(yù)期結(jié)果一致。試劑盒操作方便。

By 王老師

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

A:
Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit

If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!