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  4. Mouse D-Lactate Dehydrogenase,D-LDH ELISA Kit

Mouse D-Lactate Dehydrogenase,D-LDH ELISA Kit

  • 中文名稱：
    小鼠D-乳酸脫氫酶(D-LDH)酶聯免疫試劑盒
  • 貨號：
    CSB-E11723m
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠D-乳酸脫氫酶(D-LDH)酶聯免疫試劑盒（CSB-E11723m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的LDHD含量。試劑盒檢測范圍為4.69 mU/mL-300 mU/mL，適用于科研領域中對小鼠模型中D-LDH功能的研究，例如腸道缺血再灌注損傷機制探索、糖尿病相關代謝異常評估或細胞能量代謝實驗等場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Ldhd ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit; mitochondrial ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; EC 1.1.2.4 ELISA Kit
  • 縮寫：
    LDHD
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    4.69 mU/mL-300 mU/mL
  • 靈敏度：
    1.17 mU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-89  
    1:2 Average % 93  
    Range % 90-96  
    1:4 Average % 87  
    Range % 83-90  
    1:8 Average % 98  
    Range % 94-102  
  • 回收率：
    The recovery of mouse D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-95  
    EDTA plasma (n=4) 104 101-107  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected  
    300 2.278 2.304 2.291 2.111  
    150 1.422 1.500 1.461 1.281  
    75 0.932 0.921 0.927 0.747  
    37.5 0.623 0.624 0.624 0.444  
    18.75 0.385 0.394 0.390 0.210  
    9.38 0.287 0.275 0.281 0.101  
    4.69 0.242 0.231 0.237 0.057  
    0 0.181 0.179 0.180    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Involved in D-lactate, but not L-lactate catabolic process.
  • 基因功能參考文獻：
    1. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
  • 亞細胞定位：
    Mitochondrion.
  • 蛋白家族：
    FAD-binding oxidoreductase/transferase type 4 family
  • 組織特異性：
    Readily detected in liver and kidney, with a weaker signal observed in heart, skeletal muscle, stomach, brain, and lung.
  • 數據庫鏈接：