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Mouse Apolipoprotein C-II(APOC2) ELISA kit

  • 中文名稱(chēng):
    小鼠載脂蛋白C-II(APOC2)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL001932MO
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 促銷(xiāo):
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠載脂蛋白C-II(APOC2)酶聯(lián)免疫試劑盒(CSB-EL001932MO)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的APOC2含量。APOC2即載脂蛋白C2,它在脂質(zhì)代謝中發(fā)揮重要作用,是脂蛋白脂肪酶(LPL)的激活劑,可促進(jìn)乳糜微粒和極低密度脂蛋白的分解代謝。目前關(guān)于APOC2的研究,多圍繞其在脂質(zhì)代謝異常相關(guān)疾病如高脂血癥等中的機(jī)制,探索其作為治療靶點(diǎn)的潛力。試劑盒檢測(cè)范圍為18.8 ng/mL-1200 ng/mL,科研人員可通過(guò)研究脂代謝異常相關(guān)疾?。ㄈ绶逝?、糖尿病)的分子機(jī)制,或評(píng)估藥物干預(yù)對(duì)脂代謝途徑的影響;為心血管疾病及代謝綜合征的基礎(chǔ)研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Apoc2Apolipoprotein C-II ELISA kit; Apo-CII ELISA kit; ApoC-II ELISA kit; Apolipoprotein C2 ELISA kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    18.8 ng/mL-1200 ng/mL
  • 靈敏度:
    9.4 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 97  
    Range % 88-102  
    1:40 Average % 93  
    Range % 85-98  
    1:80 Average % 103  
    Range % 96-107  
    1:160 Average % 104  
    Range % 95-109  
  • 回收率:
    The recovery of mouse APOC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 91-99  
    EDTA plasma (n=4) 101 94-107  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    1200 0.094 0.097 0.096    
    600 0.229 0.221 0.225    
    300 0.429 0.444 0.437    
    150 0.707 0.726 0.717    
    75 1.109 1.068 1.089    
    37.5 1.535 1.474 1.505    
    18.8 1.682 1.639 1.661    
    0 2.048 1.967 2.008    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase.
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Apolipoprotein C2 family
  • 組織特異性:
    Adult and fetal liver, intestine and peritoneal macrophages.
  • 數(shù)據(jù)庫(kù)鏈接: