Human oxytocin,OT ELISA Kit

產(chǎn)品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人催產(chǎn)素(OT)酶聯(lián)免疫試劑盒（CSB-E08994h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的OT含量。人催產(chǎn)素（OT）是一種哺乳動物激素，由9個氨基酸構(gòu)成，主要在大腦下視丘分泌，參與分娩、泌乳和母嬰關(guān)系等。其機制研究顯示，OT通過調(diào)節(jié)神經(jīng)遞質(zhì)和受體基因，影響信任、合作和社會認知等行為，具有臨床應(yīng)用潛力，如治療自閉癥和社交恐懼癥。試劑盒檢測范圍為8 μIU/mL-400 μIU/mL，適用于基礎(chǔ)科研中探索神經(jīng)生物學機制、行為學關(guān)聯(lián)性分析或母嬰健康相關(guān)研究；該產(chǎn)品為神經(jīng)科學、心理學及內(nèi)分泌學領(lǐng)域的研究者提供可靠工具，用于定量分析生物樣本中OT的動態(tài)變化，支持探索其在生理調(diào)控及疾病模型中的潛在作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

OT
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

8 μIU/mL-400 μIU/mL
靈敏度：

10 μIU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-96
1:2	Average %	95
	Range %	91-99
1:4	Average %	98
	Range %	92-103
1:8	Average %	93
	Range %	87-98

回收率：

The recovery of human OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	96	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.