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  4. Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

  • 中文名稱：
    人細胞角蛋白21-1片段(CYFRA21-1)酶聯免疫試劑盒
  • 貨號：
    CSB-E09606h
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人細胞角蛋白21-1片段(CYFRA21-1)酶聯免疫試劑盒（CSB-E09606h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的CYFRA21-1含量。CYFRA21-1是一種細胞角蛋白片段，廣泛分布于正常組織中。當細胞癌變時，角蛋白被降解并釋放到血液中，CYFRA21-1水平升高。它作為肺癌，尤其是鱗狀細胞癌的標志物，可用于診斷和預后評估。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，本產品適用于基礎醫學研究中腫瘤標志物表達水平檢測、疾病機制探索或藥物開發過程中的生物標志物動態監測，為體外實驗提供血清/血漿樣本中CYFRA21 - 1濃度的可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    CYFRA21-1
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %82-90
    1:2Average %98
    Range %91-105
    1:4Average %99
    Range %94-110
    1:8Average %92
    Range %86-99
  • 回收率：
    The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8980-98
    EDTA plasma (n=4)9790-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.541 2.385 2.463 2.305
    102.229 2.082 2.156 1.998
    51.607 1.545 1.576 1.418
    2.50.866 0.842 0.854 0.696
    1.250.463 0.439 0.451 0.293
    0.6250.337 0.324 0.331 0.173
    0.3120.254 0.246 0.250 0.092
    00.160 0.156 0.158 　
  • 數據處理：
  • 貨期：
    3-5 working days

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相關問答

■ 常見問題解答
Q:

May I know are the antibodies used in the ELISA kit polyclonal antibodies or monoclonal antibodies?
Human cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit

A:
Thanks for your inquiry.Pls check the details as below:
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody
If further questions,pls let me know.