Human Small proline-rich protein 2B(SPRR2B) ELISA kit

產(chǎn)品詳情

說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人小富脯氨酸蛋白2B(SPRR2B)酶聯(lián)免疫試劑盒（CSB-EL022613HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SPRR2B含量。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，支持科研人員在基礎(chǔ)研究中探索SPRR2B在皮膚生理病理過(guò)程中的作用機(jī)制，例如表皮分化異常、皮膚炎癥模型或相關(guān)疾病分子調(diào)控研究，為體外實(shí)驗(yàn)提供可靠的蛋白表達(dá)定量數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

SPRR2B ELISA Kit; Small proline-rich protein 2B ELISA Kit; SPR-2B ELISA Kit
縮寫(xiě)：

SPRR2B
Uniprot No.：

P35325
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SPRR2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	103
	Range %	99-105
1:4	Average %	93
	Range %	87-97
1:8	Average %	91
	Range %	88-96

回收率：

The recovery of human SPRR2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-101
EDTA plasma (n=4)	90	86-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane.
基因功能參考文獻(xiàn)：
1. Knockdown of the Sprr2b gene or inhibition of SPRR2B phosphorylation attenuates USP7/MDM2 binding and p53 degradation, leading to fibroblasts cell cycle arrest. PMID: 29581288
2. An SNP in SPRR2B was predictive of asthma among white children with eczema from 2 independent populations. PMID: 22374195
3. We showed that just four genes, G3BP2, SCARB2, CSNK1A1 and SPRR2B, can classify patients as presence of lymph node metastasis negative or positive, with 80.0% accuracy. PMID: 21985131
4. SPR2B transcripts and protein were moderately increased in the human papillomaviurs 11- and 59-infected tissues. PMID: 14748073
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Cornifin (SPRR) family
組織特異性：

Suprabasal layers of squamous-differentiated tissues such as epidermis, esophagus, tongue and trachea.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 11262

OMIM: 182268

KEGG: hsa:6701

STRING: 9606.ENSP00000340703

UniGene: Hs.568239