Human Protein FAM132B(FAM132B) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人FAM132B蛋白(FAM132B)酶聯(lián)免疫試劑盒（CSB-EL008059HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的FAM132B含量。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于體外培養(yǎng)細(xì)胞模型的功能研究、動(dòng)物實(shí)驗(yàn)樣本的分子機(jī)制探索，以及臨床前研究中生物標(biāo)志物的定量分析等科研場(chǎng)景，為代謝調(diào)控通路、細(xì)胞分化機(jī)制等基礎(chǔ)研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

Complement C1q tumor necrosis factor-related protein 15 ELISA Kit; ERFE_HUMAN ELISA Kit; Erythroferrone ELISA Kit; FAM132B ELISA Kit; Myonectin ELISA Kit
縮寫：

FAM132B
Uniprot No.：

Q4G0M1
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cell Biology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-95
1:2	Average %	95
	Range %	90-101
1:4	Average %	87
	Range %	83-94
1:8	Average %	99
	Range %	94-105

回收率：

The recovery of human FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-102
EDTA plasma (n=4)	88	84-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
基因功能參考文獻(xiàn)：
1. From a clinical point of view, erythroferrone could become a useful biological marker of iron metabolism and a therapeutic target. [review] PMID: 28666715
2. FAM132b expression is upregulated in Congenital Dyserythropoietic Anemia type II. PMID: 27540014
3. In hemodialysis patients DA and CERA increased levels of ERFE that regulated hepcidin 25 and led to iron mobilization from body stores during erythropoiesis. PMID: 26978524
4. Myonectin links skeletal muscle to lipid homeostasis in liver and adipose tissue in response to alterations in energy state, revealing a novel myonectin-mediated metabolic circuit. PMID: 22351773
亞細(xì)胞定位：

Secreted.
蛋白家族：

Adipolin/erythroferrone family
數(shù)據(jù)庫(kù)鏈接：

HGNC: 26727

OMIM: 615099

KEGG: hsa:151176

UniGene: Hs.24951