Human Pancreastatin ELISA Kit

Human Pancreastatin ELISA Kit

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文獻(xiàn)引用 (1) 說(shuō)明書下載

中文名稱：

人胰抑制素(Pancreastatin)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E09209h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
促銷：
其他：

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產(chǎn)品描述：

人胰抑制素(Pancreastatin)酶聯(lián)免疫試劑盒（CSB-E09209h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Pancreastatin含量。人胰抑制素（Pancreastatin）是一種由胰島β細(xì)胞分泌的肽類激素，與胰島素協(xié)同調(diào)節(jié)血糖穩(wěn)態(tài)。其研究顯示，人胰抑制素能延緩胃排空、降低餐后血糖，并通過(guò)調(diào)節(jié)胰島素和胰高血糖素的分泌來(lái)維持血糖平衡。其作用機(jī)制涉及影響胰島素分泌、調(diào)節(jié)血糖和脂肪代謝等方面。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，科研人員可將其應(yīng)用于探究Pancreastatin在糖尿病、肥胖癥等代謝異常病理機(jī)制中的作用，或用于評(píng)估藥物干預(yù)、基因編輯等實(shí)驗(yàn)?zāi)Ｐ椭性摰鞍椎膭?dòng)態(tài)表達(dá)水平變化，為相關(guān)基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

Pancreastatin
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human pancreastatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-110
1:2	Average %	97
	Range %	91-105
1:4	Average %	87
	Range %	82-93
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human pancreastatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	90	85-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.439	2.352	2.396	2.267
1000	1.808	1.676	1.742	1.613
500	1.219	1.184	1.202	1.073
250	0.704	0.739	0.722	0.593
125	0.413	0.424	0.419	0.290
62.5	0.317	0.329	0.323	0.194
31.25	0.234	0.229	0.232	0.103
0	0.131	0.127	0.129

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Role of chromogranin A-derived fragments after resection of nonfunctioning pancreatic neuroendocrine tumors Andreasi V,J Endocrinol Invest,2022

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.