Human Immunoglobulin G4 (IgG4) ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人免疫球蛋白G4(IgG4)酶聯免疫試劑盒（CSB-E13728h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgG4含量。IgG4是一種免疫球蛋白，在免疫調節中發揮獨特作用。其背景上，IgG4異常與多種疾病相關。研究機制方面，它親和力成熟高，能發生Fab臂交換形成不對稱抗體，可阻斷抗原抗體相互作用，目前在自身免疫病、腫瘤等研究中受關注。試劑盒檢測范圍為39 ng/mL-10000 ng/mL，該產品適用于科研領域中對IgG4表達水平的體外研究，例如探索其在免疫調節、過敏反應或纖維化疾病中的生物學作用，也可作為評估實驗模型中免疫應答特征的輔助工具，為炎癥機制研究、生物標志物篩選及藥物開發提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgG4
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

39 ng/mL-10000 ng/mL
靈敏度：

39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IgG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	91-101
1:2000	Average %	99
	Range %	93-104
1:4000	Average %	92
	Range %	87-96
1:8000	Average %	90
	Range %	85-94

回收率：

The recovery of human IgG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	93	88-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.