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Human 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit

  • 中文名稱:
    人6酮前列腺素F1a(6-keto-PGF1a)酶聯免疫試劑盒
  • 貨號:
    CSB-E09420h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人6酮前列腺素F1a(6-keto-PGF1a)酶聯免疫試劑盒(CSB-E09420h)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的6ketoPGF1a含量。人6酮前列腺素F1a(6-keto-PGF1a)是一種由前列腺素I2(PGI2)代謝生成的穩定產物,其在人體內具有調節血管功能和血小板聚集的作用。6-keto-PGF1a的研究背景主要涉及其在心血管疾病、炎癥反應和腫瘤發生發展中的作用機制。試劑盒檢測范圍為50 pg/mL-800 pg/mL,適用于心血管疾病機制研究、藥物開發中前列環素代謝評估以及內皮功能相關實驗,可為血管生物學、藥理學及病理生理學研究提供高效分析工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    6ketoPGF1a
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    50 pg/mL-800 pg/mL
  • 靈敏度:
    50 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human 6-K-PGF1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 87-99  
    1:2 Average % 97  
    Range % 93-105  
    1:4 Average % 85  
    Range % 82-89  
    1:8 Average % 90  
    Range % 85-98  
  • 回收率:
    The recovery of human 6-K-PGF1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-95  
    EDTA plasma (n=4) 95 89-99  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    800 0.218 0.211 0.215    
    400 0.350 0.333 0.342    
    200 0.603 0.612 0.608    
    100 1.111 1.102 1.107    
    50 2.219 2.178 2.199    
  • 數據處理:
  • 貨期:
    3-5 working days

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