Hamster Interleukin 6,IL-6 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

倉鼠白介素6(IL-6)酶聯(lián)免疫試劑盒（CSB-E14304Ha）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL-6含量。IL-6是重要細(xì)胞因子，在免疫調(diào)節(jié)、炎癥反應(yīng)等生理病理過程中起關(guān)鍵作用。其過度表達(dá)與多種疾病如類風(fēng)濕關(guān)節(jié)炎、新冠肺炎等密切相關(guān)。研究機(jī)制主要圍繞抑制IL-6及其受體信號通路，以調(diào)控炎癥反應(yīng)和細(xì)胞增殖等，為疾病治療提供方向。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，科研人員可將其應(yīng)用于炎癥機(jī)制研究、免疫治療模型評估或藥物研發(fā)中的細(xì)胞因子動態(tài)監(jiān)測，特別適合體外實驗環(huán)境下的定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-6
種屬：

Mesocricetus auratus (Golden hamster)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	95
	Range %	91-99
1:4	Average %	96
	Range %	91-100
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of hamster IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-104
EDTA plasma (n=4)	91	85-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.