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  4. Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

  • 中文名稱：
    魚(yú)催乳素(PRL/LTH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12695Fh
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚(yú)催乳素(PRL/LTH)酶聯(lián)免疫試劑盒（CSB-E12695Fh）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PRL/LTH含量。PRL（泌乳素）和LTH（促乳素，常指PRL）在人體內(nèi)分泌調(diào)節(jié)中起關(guān)鍵作用，與乳腺發(fā)育和泌乳等生理過(guò)程相關(guān)。研究聚焦于其在疾病中的機(jī)制，如高泌乳素血癥發(fā)病機(jī)制、藥物對(duì)其水平的影響及信號(hào)通路探究，為相關(guān)疾病治療提供方向。試劑盒檢測(cè)范圍為10 μIU/mL-4000 μIU/mL，廣泛應(yīng)用于魚(yú)類繁殖生理學(xué)研究、環(huán)境內(nèi)分泌干擾物評(píng)估、水產(chǎn)養(yǎng)殖過(guò)程中激素水平監(jiān)測(cè)等科研場(chǎng)景，為水生生物內(nèi)分泌機(jī)制探索提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    prl1 ELISA Kit; Prolactin-1 ELISA Kit; PRL-188 ELISA Kit; Prolactin I ELISA Kit
  • 縮寫：
    PRL/LTH
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    10 μIU/mL-4000 μIU/mL
  • 靈敏度：
    10 μIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-100  
    1:2 Average % 95  
    Range % 86-104  
    1:4 Average % 90  
    Range % 85-95  
    1:8 Average % 96  
    Range % 92-108  
  • 回收率：
    The recovery of fish PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-102  
    EDTA plasma (n=4) 95 88-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average 　  
    4000 0.350 0.364 0.357 　  
    750 0.697 0.705 0.701 　  
    125 1.196 1.203 1.200 　  
    35 1.523 1.519 1.521 　  
    10 1.700 1.707 1.704 　  
    0 2.244 2.242 2.243 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Which fish species / family is it optimized for? We work with a non-model species and would like to check its reactivity. Thank you.

A:
Thanks for your inquiry.
CSB-E12695Fh Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit
This kit is designed against salmon. There is no requirement for fish species. Gnerally all kinds of fish can be detected out. We suggest you do a pretest.
Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫(kù)鏈接：

    UniGene: Oni.44217