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Your Good Partner in Biology Research

Dog insulin-like growth factor binding protein 3 (IGFBP3)ELISA kit

  • 中文名稱:
    犬胰島素樣生長因子結合蛋白3(IGFBP3)酶聯免疫試劑盒
  • 貨號:
    CSB-EL011097DO
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    犬胰島素樣生長因子結合蛋白3(IGFBP3)酶聯免疫試劑盒(CSB-EL011097DO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IGFBP3含量。IGFBP3是一種重要靶點。它參與胰島素樣生長因子(IGF)信號通路的調控,在細胞的生長、增殖、凋亡等過程中發揮作用。相關研究機制主要聚焦于其與IGF的結合,以及對下游信號傳導途徑的影響,為腫瘤等疾病的治療提供新方向。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL,本試劑盒適用于科研場景中犬類生長發育研究、代謝紊亂模型建立、內分泌調控機制探索等領域,例如通過檢測血清IGFBP3水平評估生長激素軸功能狀態,或分析營養干預對組織IGF信號通路的影響。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    3.12 ng/mL-200 ng/mL
  • 靈敏度:
    0.78 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IGFBP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:20Average %94
    Range %91-99
    1:40Average %96
    Range %90-100
    1:80Average %97
    Range %95-100
    1:160Average %95
    Range %89-103
  • 回收率:
    The recovery of dog IGFBP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-98
    EDTA plasma (n=4)9690-102
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.958 2.943 2.951 2.775
    1002.120 2.131 2.126 1.950
    501.074 1.063 1.069 0.893
    250.575 0.562 0.569 0.393
    12.50.343 0.349 0.346 0.170
    6.250.268 0.274 0.271 0.095
    3.120.209 0.224 0.217 0.041
    00.177 0.175 0.176  
  • 數據處理:
  • 貨期:
    3-5 working days

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