Bovine Immunoglobulin A,IgA ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛免疫球蛋白A(IgA)酶聯免疫試劑盒（CSB-E12018B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IgA含量。IgA 即免疫球蛋白 A，是人體免疫系統的重要組成部分，在黏膜免疫中發揮關鍵作用。研究圍繞其結構與功能展開，探索其識別病原體機制，以及在免疫調節中的角色。IgA 相關研究為感染性疾病、自身免疫疾病診斷與治療帶來新方向。試劑盒檢測范圍為0.39 μg/mL-25 μg/mL，適用于研究牛群免疫狀態、疫苗效力評估及腸道健康機制等科研領域；畜牧免疫學研究、疫苗開發驗證、病原感染模型建立等場景；為牛免疫相關基礎研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgA
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.39 μg/mL-25 μg/mL
靈敏度：

0.39 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-99
1:200	Average %	96
	Range %	92-101
1:400	Average %	85
	Range %	81-87
1:800	Average %	89
	Range %	83-94

回收率：

The recovery of bovine IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	97	92-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.