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ROBO1

ROBO1,全稱為Roundabout homolog 1,在神經(jīng)發(fā)育和癌癥中扮演著重要角色。它最初在果蠅中被發(fā)現(xiàn),與Slit2配體共同作用,引導(dǎo)神經(jīng)軸突生長方向,防止其跨過體軸中線形成紊亂。ROBO1蛋白也被稱為Dutt1,與多種癌癥的發(fā)生發(fā)展相關(guān),如肝癌和結(jié)直腸癌。研究發(fā)現(xiàn),ROBO1蛋白在肝癌細(xì)胞中常有過表達(dá)現(xiàn)象,且其表達(dá)水平與腫瘤的分期和級別無明顯關(guān)聯(lián)。此外,ROBO1蛋白在乳腺癌和腎細(xì)胞癌中也會被高度甲基化,提示其可能是一個抑癌基因。然而,在肝癌中,ROBO1蛋白卻呈現(xiàn)過表達(dá)狀態(tài),這可能與腫瘤微環(huán)境中的信號通路變化有關(guān)。目前,針對ROBO1蛋白的藥物研發(fā)尚處于早期階段,但已有研究探索其作為潛在治療靶點(diǎn)的可能性。例如,有研究通過RNA干擾技術(shù)敲低ROBO1蛋白表達(dá),發(fā)現(xiàn)可以顯著抑制肝癌細(xì)胞的遷移和侵襲能力,并降低其生長速度和轉(zhuǎn)移能力。這些發(fā)現(xiàn)提示ROBO1蛋白可能成為肝癌治療的新靶點(diǎn),值得進(jìn)一步研究。

熱銷產(chǎn)品

Recombinant Human Roundabout homolog 1 (ROBO1), partial (Active) (CSB-MP896760HU)

驗(yàn)證數(shù)據(jù)

CSB-MP896760HU

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CSB-MP896760HU

Activity
Measured by its binding ability in a functional ELISA.Immobilized Human ROBO1 at 2 μg/ml can bind Anti-ROBO1 recombinant antibody (CSB-RA896760MA1HU).The EC50 is 0.7363-0.9105 ng/mL.

CSB-MP896760HU

The purity of ROBO1 was greater than 90% as determined by SEC-HPLC

ROBO1 Recombinant Monoclonal Antibody (CSB-RA896760MA1HU)

驗(yàn)證數(shù)據(jù)

CSB-RA896760MA1HU

IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

CSB-RA896760MA1HU

IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

CSB-RA896760MA1HU

Immunofluorescence staining of A549 cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-RA896760MA1HU

Immunofluorescence staining of Hela cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-RA896760MA1HU

Overlay Peak curve showing Hela cells surface stained with CSB-RA896760MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,025 events was performed.

ROBO1 Antibody (CSB-PA896760LA01HU)

驗(yàn)證數(shù)據(jù)

CSB-PA896760LA01HU

Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: ROBO1 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 181, 182, 177, 176, 170 kDa
Observed band size: 181 kDa

CSB-PA896760LA01HU

Immunofluorescent analysis of Hela cells using CSB-PA896760LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Human Roundabout homolog 1(ROBO1) ELISA kit (CSB-EL020054HU)

驗(yàn)證數(shù)據(jù)

CSB-EL020054HU 標(biāo)準(zhǔn)曲線

貨號:CSB-EL020054HU

規(guī)格:96T/48T

靈敏度:0.039 ng/mL

檢測范圍:0.156 ng/mL-10 ng/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ROBO1 Antibodies

ROBO1 for Homo sapiens (Human)

ROBO1 Proteins

ROBO1 Proteins for Homo sapiens (Human)

ROBO1 ELISA Kit

ROBO1 ELISA Kit for Homo sapiens (Human)