ROBO1
ROBO1,全稱為Roundabout homolog 1,在神經(jīng)發(fā)育和癌癥中扮演著重要角色。它最初在果蠅中被發(fā)現(xiàn),與Slit2配體共同作用,引導(dǎo)神經(jīng)軸突生長方向,防止其跨過體軸中線形成紊亂。ROBO1蛋白也被稱為Dutt1,與多種癌癥的發(fā)生發(fā)展相關(guān),如肝癌和結(jié)直腸癌。研究發(fā)現(xiàn),ROBO1蛋白在肝癌細(xì)胞中常有過表達(dá)現(xiàn)象,且其表達(dá)水平與腫瘤的分期和級別無明顯關(guān)聯(lián)。此外,ROBO1蛋白在乳腺癌和腎細(xì)胞癌中也會被高度甲基化,提示其可能是一個抑癌基因。然而,在肝癌中,ROBO1蛋白卻呈現(xiàn)過表達(dá)狀態(tài),這可能與腫瘤微環(huán)境中的信號通路變化有關(guān)。目前,針對ROBO1蛋白的藥物研發(fā)尚處于早期階段,但已有研究探索其作為潛在治療靶點(diǎn)的可能性。例如,有研究通過RNA干擾技術(shù)敲低ROBO1蛋白表達(dá),發(fā)現(xiàn)可以顯著抑制肝癌細(xì)胞的遷移和侵襲能力,并降低其生長速度和轉(zhuǎn)移能力。這些發(fā)現(xiàn)提示ROBO1蛋白可能成為肝癌治療的新靶點(diǎn),值得進(jìn)一步研究。
熱銷產(chǎn)品
Recombinant Human Roundabout homolog 1 (ROBO1), partial (Active) (CSB-MP896760HU)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human ROBO1 at 2 μg/ml can bind Anti-ROBO1 recombinant antibody (CSB-RA896760MA1HU).The EC50 is 0.7363-0.9105 ng/mL.
The purity of ROBO1 was greater than 90% as determined by SEC-HPLC
ROBO1 Recombinant Monoclonal Antibody (CSB-RA896760MA1HU)
驗(yàn)證數(shù)據(jù)
IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A549 cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of Hela cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay Peak curve showing Hela cells surface stained with CSB-RA896760MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,025 events was performed.
ROBO1 Antibody (CSB-PA896760LA01HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: ROBO1 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 181, 182, 177, 176, 170 kDa
Observed band size: 181 kDa
Immunofluorescent analysis of Hela cells using CSB-PA896760LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Human Roundabout homolog 1(ROBO1) ELISA kit (CSB-EL020054HU)
驗(yàn)證數(shù)據(jù)
貨號:CSB-EL020054HU
規(guī)格:96T/48T
靈敏度:0.039 ng/mL
檢測范圍:0.156 ng/mL-10 ng/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ROBO1 Antibodies
ROBO1 for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA020054GA01HU | ROBO1 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA896760LA01HU | ROBO1 Antibody | Human | ELISA, WB, IF |
| CSB-PA896760LB01HU | ROBO1 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA896760LC01HU | ROBO1 Antibody, FITC conjugated | Human | |
| CSB-PA896760LD01HU | ROBO1 Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA896760MA1HU | ROBO1 Recombinant Monoclonal Antibody | Human | ELISA, IHC, IF, FC |
ROBO1 Proteins
ROBO1 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP896760HU CSB-EP896760HU CSB-BP896760HU CSB-EP896760HU-B |
Recombinant Human Roundabout homolog 1 (ROBO1), partial | Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
| CSB-MP896760HU | Recombinant Human Roundabout homolog 1 (ROBO1), partial (Active) | Mammalian cell |
ROBO1 ELISA Kit
ROBO1 ELISA Kit for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL020054HU | Human Roundabout homolog 1(ROBO1) ELISA kit | serum, plasma, tissue homogenates | 0.039 ng/mL |






